Improvement of single-cell gel electrophoresis (SCGE) alkaline comet assay

Improvement of single-cell gel electrophoresis (SCGE) alkaline comet assay

Although the single-cell gel electrophoresis (SCGE) alkaline comet assay can detect DNA damage quickly and with high sensitivity, it does not work well for cryopreserved sperm of some marine teleosts. Using large yellow croaker Pseudosciaena crocea sperm that were cryopreserved in increasing concentrations (5 to 30%) of DMSO, we made modifications to the classic SCGE method that included using ...

Single Cell Gell Electrophoresis (Comet Assay)

DRAFT OECD GUIDELINE FOR THE TESTING OF CHEMICALS Rodent alkaline single cell gel electrophoresis (Comet) assay INTRODUCTION

1. OECD Test Guidelines (TGs) are available for a wide range of in vitro genotoxicity assays that are able to detect DNA damage, gene mutation and/or chromosomal aberrations. There are TGs for in vivo endpoints (i.e. chromosomal aberrations, gene mutations and DNA repair as unscheduled DNA synthesis); however, these do not directly measure DNA damage. The alkaline single cell gel electrophoresi...

Genotoxicity of select herbicides in Rana catesbeiana tadpoles using the alkaline single-cell gel DNA electrophoresis (comet) assay.

Pesticides are broadly used for pest control in agriculture despite possible negative impacts they may pose to the environment. Thus, we examined the DNA damage caused by five herbicides commonly used in southern Ontario (Canada). Erythrocytes from Rana catesbeiana (bullfrog) tadpoles were evaluated for DNA damage following exposure to selected herbicides, using the alkaline single-cell gel DNA...

Comparison of sensitivity to methyl methanesulphonate among tadpole developmental stages using the alkaline single-cell gel electrophoresis (comet) assay.

In a previous study, we demonstrated that tadpoles are suitable organisms for monitoring small bodies of water (e.g., creeks, ponds, and drainage ditches) for genotoxicity using the alkaline single-cell gel DNA electrophoresis (SCG) or "comet" assay [Ralph and Petras, 1997]. This approach involves detection, under alkaline conditions, of cell DNA fragments which on electrophoresis migrate from ...